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1. was proven to be ineffective for sequencing genomes of complex organisms and is not used in Next Generation Sequencing approaches. 2. was used to generate the first genome sequence of Haemophilus influenzae. 3. was used by the Celera group to generate the first genome sequence of Drosophila melanogaster. 4. was demonstrated by Hood’s group in 1995 to be a valid approach to sequence a genome.

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1. was proven to be ineffective for sequencing genomes of complex organisms and is not used in Next Generation Sequencing approaches.
 2. was used to generate the first genome sequence of Haemophilus influenzae.
3. was used by the Celera group to generate the first genome sequence of Drosophila melanogaster.
4. was demonstrated by Hood’s group in 1995 to be a valid approach to sequence a genome.
 
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