Amino acid
Question
Amino acid sequence analysis of all of the peptides found in a single IgG antibody would reveal unique peptide
sequences totaling ~600–700 amino acids. Using this estimate, the predicted molecular weight of an antibody protein would be ~70–75 kDa. Yet, an intact antibody protein has a molecular weight of ~150 kDa. The explanation for this discrepancy is:
| Each IgG antibody is a complex of four identical polypeptides. |
| IgG antibodies have many more heavy amino acids in them than most other proteins |
| IgG antibodies tend to aggregate together during purification, thereby distorting molecular weight estimates |
| IgG antibodies are produced as dimers of two identical IgG monomers |
| Each IgG antibody is a complex of two identical light chains and two identical heavy chains |
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