Entries by Hannah Wangui

transformation experiment

If you set up a transformation experiment in which you mixed competent E. Coli with a new plasmid that carried a gene for GFP, but not a gene for ampicillin-resistance, what colonies would you expect to grow on an LB plate with arabinose, and no ampicillin? a. fluorescent green colonies only b. white colonies only […]

 

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What are some factors that lead to individual variability in RFLPs (restriction fragment length polymorphism)?

What are some factors that lead to individual variability in RFLPs (restriction fragment length polymorphism)?   Looking for a Similar Assignment? Order now and Get 10% Discount! Use Coupon Code “Newclient”

 

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antibiotic resistance genes

A plasmid that carries two antibiotic resistance genes, to ampicillin and tetracycline, respectively, has one restriction site for EcoRI located within the tetracycline resistance gene. Eukaryotic DNA containing a gene of interest (g.o.i) has been digested with different restriction enzymes, none of which cut within the gene of interest sequence). The resultant fragments are added […]

 

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