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DNA

You are characterizing a sequence-specific DNA binding protein whose

molecular weight is 20kd, and it ends in one sequence very specifically. You are trying to set up a protein: DNA complex such that all the protein in the solution is bound to the DNA. You are using plasmid DNA containing 2500 base pairs that have one recognition sequence for this protein. one base pair has a molecular weight of 660 daltons. If you have 20 poles of the protein, how many micrograms of plasmid DNA would you need to add to the reaction in order to have all the protein bound to DNA?

 
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