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If no bands present on the agarose gel after addition of fluorescent dye, does it mean that there is no others pathogen in the unknown sample? PCR detection of water-borne pathogens?

If no bands present on the agarose gel after addition of fluorescent
dye, does it mean that there is no others pathogen in the unknown sample? PCR detection of water-borne pathogens? please provide atleast 5 reasons

 
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