Question 1: For the following double-stranded DNA sequence,

-CATTGACCGTAA-

-GTAACTGGCATT-

Answer the following questions:

• Assume that RNA polymerase will read the top strand of DNA as the “template” to synthesize mRNA. What will be the sequence of the mRNA synthesized? 

• Based upon the mRNA sequence above, what is the peptide sequence?  

If there was a mutation in the DNA sequence above resulting in the following DNA sequence,

-GATTGACCGTAA-

-CTAACTGGCATT-

• What would be the resulting mRNA, assuming RNA polymerase will use the DNA sequence, -GATTGACCGTAA- ? 

• Based upon the mRNA sequence above, what is the peptide sequence? 

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