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Question Hello! Solutions containing the amino acids tryptophan and tyrosine can be analyzed under alkaline conditions from their different UV spectra. The molar extinction coefficients under these conditions at 240 nm and 280 nm are: Tyrosine:e240= 11,300 M-1cm-1 e280= 1,500 M-1cm-1 Tryptophan:e240= 1,960 M-1cm-1 e280= 5,380 M-1cm-1 The absorbance (1 cm optical pathlength) of a solution of tryptophan and tyrosine is measured to be 0.717 at 240 nm and 0.239 at 280 nm. Determine the concentration of tyrosine in units of millimolar(mM).

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Question

Hello!Solutions containing the amino acids tryptophan and tyrosine can be analyzed under alkaline

conditions from their different UV spectra. The molar extinction coefficients under these conditions at 240 nm and 280 nm are:

Tyrosine:e240= 11,300 M-1cm-1

e280= 1,500 M-1cm-1

Tryptophan:e240= 1,960 M-1cm-1

e280= 5,380 M-1cm-1

The absorbance (1 cm optical pathlength) of a solution of tryptophan and tyrosine is measured to be 0.717 at 240 nm and 0.239 at 280 nm.

Determine the concentration of tyrosine in units of millimolar(mM).

 
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