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Question The attached is my SDS_PAGE gel of rGFP. The samples from left to right are G0, G3, GCE, W2, W3, E2, E3, and MW ladder. My question is how to evaluate the quantity of this gel? For each sample, I see lot of different bands, how to estimate the molecular weight of each sample, and how to determine the migration time? Given the MW of the ladder are 97.4, 66.2, 45, 31, 21.5, 14.4 kD. Thank you

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Question

The attached is my SDS_PAGE gel of rGFP. The samples from left to right are G0, G3, GCE, W2, W3, E2, E3, and MW

ladder. My question is how to evaluate the quantity of this gel? For each sample, I see lot of different bands, how to estimate the molecular weight of each sample, and how to determine the migration time? Given the MW of the ladder are 97.4, 66.2, 45, 31, 21.5, 14.4 kD.

Thank you

 
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