You are a researcher working in a molecular biology laboratory. You need to run a batch of PCRs (polymerase chain reaction) to verify the presence of a specific gene in the organism you are currently working on. 1) What are all the necessary reagents required for the PCR (hint=5)? 2) After you assemble your reaction in the PCR tubes this goes into the thermocycler which will control the temperature necessary for each main step to occur. How are those steps called and what is their main function? 3) After the PCR program ran to completion, you performed a gel electrophoresis. What is the function of this step?
You are a researcher working in a molecular biology laboratory. You need to run a batch of PCRs
(polymerase chain reaction) to verify the presence of a specific gene in the organism you are currently working on.
1) What are all the necessary reagents required for the PCR (hint=5)?
2) After you assemble your reaction in the PCR tubes this goes into the thermocycler which will control the temperature necessary for each main step to occur. How are those steps called and what is their main function?
3) After the PCR program ran to completion, you performed a gel electrophoresis. What is the function of this step?
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