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Meiosis and Genetic Diversity
/in Questions Uploads /by adminWhy are there two stages in meiosis? (10)
What is the main evolutionary advantage of sexual reproduction over asexual reproduction? Explain. (10)
Explain the difference between haploid and diploid. What type of cells would you expect to be haploid and which would be diploid? (10)
What is the difference between and organism’s genotype and phenotype? (10)
Explain Mendel’s Law of Segregation. (10)
Explain the different types of allele dominance and how that affects allele expression. (12.5)
One of the most important aspects of sexual reproduction is the diversity that it can have on genetic diversity. This genetic diversity is necessary for evolution. Three ways in which meiosis can contribute to genetic diversity is through crossing-over, independent assortment, and fertilization. First, explain each of these processes. Then, describe how each of these contribute to genetic diversity.
Crossing Over (12.5):
Independent Assortment (12.5):
Fertilization (12.5):
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Lab 6: Human Inheritance Lab Worksheet
Data Table 1. Phenotypes and Genotypes of Observed Trait
| Trait | Phenotype | Possible Genotypes |
| Ear Lobes (E) | ||
| Eye Color (B) | ||
| Widow’s Peak (W) | ||
| Tongue Roller (T) | ||
| Bent Little Finger (L) | ||
| Mid-Digital Hair (H) | ||
| Red Hair (R) | ||
| Curly Hair (C) | ||
| Freckles (K) | ||
| Dimples (D) | ||
| Cleft Chin (M) | ||
| Hitchhiker’s Thumb (J) | ||
| Index Finger Length (I) | ||
| Hallux Length (G) | ||
| Left-over-right Thumb (Q) |
Questions:
1. If a man does not have Hitchhiker’s thumb, what are the two possible genotypes?
2. For an individual expressing the dominant phenotype, explain why you must list two possible genotypes.
3. Are recessive traits bad for the individual? Why or why not?
4. Is the dominant trait the more common trait in the population? Why or why not?
5. You meet an individual who is unable to roll their tongue. Using your genotype(s) for tongue rolling, what are the potential phenotypic and genotypic ratios for any offspring you produce? Include a Punnett Square in your response. Note: If you express the dominant trait, you will need to perform two separate crosses.
Lab 6: Human Inheritance
Purpose
To determine personal phenotypes and genotypes for some observable traits
To determine the phenotypic and genotypic frequencies (%) of offspring
Procedure
Below are a list of commonly observed human trait. The letter that accompanies each trait is representative of the dominant allele. This is the letter you will use when recording the genotypic information for each trait.
Read through the descriptions of each trait and refer to the images provided to determine which phenotype you express. Record your phenotype in Data Table 1 on the worksheet.
Next, determine your genotype. If you express the dominant phenotype, you cannot determine the second allele you carry, so you must list the two possible genotypes. Record this in Data Table 1.
Once you have finished, answer questions 1-5 on the worksheet.
Ear lobes (E): Free ear lobes have at least one dominant allele.
People with attached earlobes are recessive.
Eye color (B): Inheritance of eye color is controlled by multiple genes, but people having the homozygous recessive genotype have blue eyes. People who have a dominant allele may have different shades of brown, hazel, or green eyes.
Widow’s Peak (W): A hairline that forms a downward point in the middle of the forehead is caused by a dominant allele. A smooth hairline is caused by a
recessive genotype.
Cleft Chin No Cleft
Tongue roller (T): A dominant allele gives some people the ability
to roll their tongues into a “U” shape when it is extended. People
with the recessive alleles cannot roll their tongues.
Bent Little Finger (L): A dominant allele results in the end joint of the little finger of each hand bending inward. Straight little fingers are a result of the recessive genotype.
Place your hands on a flat surface, palms down, and relax. Check to see if the first joints
of your little fingers are bent or straight.
Mid-Digital Hair (H): Individuals who have hair on the middle joints of their fingers have at least one dominant allele. Those with two recessive alleles do not have hair on the joint.
Red hair (R ): Individuals with red hair have the recessive genotype. Those with any other color hair have at least one dominant allele.
Curly hair (C ): Individuals having curly hair have at least one dominant allele. People having straight hair have the recessive genotype.
Freckles (K): The recessive genotype means the individual lacks freckles. An individual with freckles will have at least one dominant allele.
Dimples (D): An individual without dimples is homozygous recessive, while an individual with dimples has at least one dominant allele.
Cleft Chin (M): An individual with a genotype of homozygous recessive will
have a cleft chin, while a person with at least one dominant allele will not have a
cleft chin.
Hitchhiker’s thumb (J): A person that can bend the last joint of the thumb to approximately a 45 degree angle has the recessive genotype while an individual that
cannot do it has at least one dominant allele.
Index Finger Length (I): If the index finger is shorter than the ring finger (4th finger next to the pinky), you have a dominant allele. If not, you have a recessive allele.
Hallux Length (G): Length of the big toe (hallux) is governed by a specific gene. Individuals with the big toe shorter in comparison to the second toe possess the dominant gene. The inheritance of a recessive genotype results in a big toe being longer than or equal to the second toe.
Left-over-right thumb crossing (Q): When the hands are folded in a natural fashion, the left thumb crosses the right thumb in a dominant genotype. If reversed, a recessive genotype is present.
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Four Current Priorities for Nursing Research
/in Questions Uploads /by adminExplain the difference between internal and external evidence. How can each of these types of evidence be utilized for quality improvement in the clinical setting?
Quantitative Research
Write a fully developed and detailed APA essay addressing each of the following points/questions. There is no required word count; be sure to completely answer all the questions for each question in detail. Separate each section in your paper with a clear heading that allows your professor to know which bullet you are addressing in that section of your paper. Support your ideas with at least one (1) source using citations in your essay. Make sure to cite using the APA writing style for the essay. The cover page and reference page are required. Review the rubric criteria for this assignment.
Conduct a literature search to select a quantitative research study related to the problem identified in Module 1 and conduct an initial critical appraisal.
(From the previous assignment submittal)
Should there be stricter guidelines of pain management that a patient should meet when using narcotics for LTACH patients?
P = LTACH Patient, I = opiod pain management, C = non-opioid pain management, O = addiction
Respond to the overview questions for the critical appraisal of quantitative studies, including:
- Is this quantitative research report a case study, case control study, cohort study, randomized control trial or systematic review?
- Where does the study fall in the hierarchy of evidence in terms of reliability and risk of bias?
- Why was the study done? (Define the problem and purpose.)
- Were the steps of the study clearly identified?
- What was the sample size?
- Are the measurements of major variables reliable and valid? Explain.
- How were the data analyzed?
- Were there any untoward events during the conduct of the study?
- How do the results fit with previous research in the area? (This may be reflected in the literature review.)
- What does this research mean to clinical practice?
Additionally, be sure to include the rapid appraisal questions for the specific research design of the quantitative study that you have chosen. These can be found in Chapter 5 of the textbook (Melnyk and Fineout-Overholt, 2015).
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See pictures and one pdf document – choose the set of questions that are needed for the type of study
This critical appraisal should be written in complete sentences (not just a numbered list) using APA format.
Provide a reference for the article according to APA format and a copy of the article.
Critical Appraisal of a Case Study
| Appraisal questions | Yes | Can’t tell |
No |
| 1. Did the study address a clearly focused question / issue? | |||
| 2. Is the research method (study design) appropriate for answering the research question? |
|||
| 3. Are both the setting and the subjects representative with regard to the population to which the findings will be referred? |
|||
| 4. Is the researcher’s perspective clearly described and taken into account? |
|||
| 5. Are the methods for collecting data clearly described? | |||
| 6. Are the methods for analyzing the data likely to be valid and reliable? Are quality control measures used? |
|||
| 7. Was the analysis repeated by more than one researcher to ensure reliability? |
|||
| 8. Are the results credible, and if so, are they relevant for practice? |
|||
| 9. Are the conclusions drawn justified by the results? | |||
| 10. Are the findings of the study transferable to other settings? |
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Natural Selection: Hardy-Weinberg
/in Questions Uploads /by adminNatural Selection: Hardy-Weinberg
Refer to the instructions that accompany this worksheet.
Materials List
- 50 red beans
- 50 white beans
- 1 paper bag or deep bowl
- 3 dishes or containers
In this activity, we will address the following question: How does natural selection affect gene frequency over several generations?
Read the instructions for the Natural Selection lab.
Hypothesis section
Specifically, what research question will this experiment test?
Hypothesize about how the frequency of the two alleles (F and f) will change over time. Explain why you expect these results.
Follow the steps for the procedure in the instructions. Record your data the data table on the following page.
Data Table
| Generation | Number of FF individuals | Number of Ff Individuals | Number of ff Individuals | Number of F alleles | Number of f alleles | Total Number of alleles | Gene Frequency of F | Gene Frequency of f |
| 1 | ||||||||
| 2 | ||||||||
| 3 | ||||||||
| 4 | ||||||||
| 5 | ||||||||
| 6 | ||||||||
| 7 | ||||||||
| 8 | ||||||||
| 9 | ||||||||
| 10 |
On the following page, insert the graph you created in Excel that shows your results.
Graph
Paste graph here
Questions:
1. Do your results support or reject your hypothesis? Explain.
2. Compare the frequencies of the dominant allele to the frequencies of the recessive allele.
3. In a real rabbit habitat new animals often come into the habitat (immigrate), and others leave the area (emigrate). How might emigration and immigration affect the gene frequency of F and f in this population of rabbits? How might you simulate this effect if you were to repeat this activity?
4. How does natural selection affect gene frequency over several generations?
5. How are the results of this simulation an example of evolution?
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Natural Selection Lab: Hardy-Weinberg
In this activity, you will examine natural selection in a small population of wild rabbits.
Evolution, on a genetic level, is a change in the frequency of alleles in a population over a period of time. Breeders of rabbits have long been familiar with a variety of genetic traits that affect the survivability of rabbits in the wild, as well as in breeding populations. One such trait is the trait for furless rabbits (naked bunnies). This trait was first discovered in England by W.E.
Castle in 1933. The furless rabbit is rarely found in the wild because the cold English winters are a definite selective force against it.
Note: In this lab, the dominant allele for normal fur is represented by F and the recessive allele for no fur is represented by f. Bunnies that inherit two F alleles (homozygous dominant) or one F and one f allele (heterozygous) have fur, while bunnies that inherit two fs (homozygous recessive) have no fur.
Procedures
- Download and open the accompanying lab worksheet. Answer the first two questions on the first page (the hypothesis section) before completing the experiment.
- The red beans represent the allele for fur (F), and the white beans represent the allele for no fur (f). The container represents the English countryside, where the rabbits randomly mate.
- Label one dish FF for the homozygous dominant genotype. Label a second dish Ff for the heterozygous genotype. Label the third dish ff for those rabbits with the homozygous recessive genotype.
- Place the 50 red and 50 white beans (alleles) in the container and shake up (mate) the rabbits. (Please note that these frequencies have been chosen arbitrarily for this activity.)
- Without looking at the beans, select two at a time, and record the results on the da ta form next to “Generation 1.” For instance, if you draw one red and one white bean, place a mark in the chart under “Number of Ff individuals.” Continue drawing pairs of beans and recording the results in your chart until all beans have been selected and sorted. Place the “rabbits” into the appropriate dish: FF, Ff, or ff. (Please note that the total number of individuals will be half the total number of beans because each rabbit requires two alleles.)
- The ff bunnies are born furless. The cold weather kills them before they reach reproductive age, so they can’t pass on their genes. Place the beans from the ff container aside before beginning the next round.
- Count the F and f alleles (beans) that were placed in each of the “furred rabbit” dishes in the first round and record the number in the chart in the columns labeled “Number of F Alleles”
and “Number of f Alleles.” (This time you are really counting each bean, but don’t count the alleles of the ff bunnies because they did not survive to reproduce.) Total the number of F alleles and f alleles for the first generation and record this number in the column labeled “Total Number of Alleles.” - Place the alleles of the surviving rabbits (which have grown, survived and reached reproductive age) back into the container and mate them again to get the next generation.
- Repeat steps five through eight to obtain generations two through ten.
- Determine the gene frequency of F and f for each generation and record them in the chart in the columns labeled “Gene Frequency of F” and “Gene Frequency of f.” To find the gene frequency of F, divide the number of F by the total, and to find the gene frequency of f, divide the number of f by the total. Express results in decimal form. The sum of the frequency of F and f should equal one for each generation.
- Graph your frequencies. Prepare a graph with the horizontal axis as the generation and the vertical axis as the frequency in decimals. Plot all frequencies on one graph. Use a solid line for F and a dashed line for f.
- Answer the remaining questions on the worksheet.
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