Question

Which of the following categories should be used in the diagnostic process in order to

decrease both the time and the cost for the following: sample analysis, viral detection, and diagnosis.

Why would this category and it’s components be the smartest/ most effective choice?

1. Direct Examination of Specimen

a. Electron and Light Microscopy

b. Antigen detection immunofluorescence, ELISA, etc.

c. Molecular techniques for the direct detection of viral genomes

2. Indirect Examination

a. Cell Culture – cytopathic effect (CPE), haemadsorption, confirmation by neutralization, interference, immunofluorescence, etc.

b. Eggs pocks on CAM – haemagglutination, inclusion bodies

c. Animals disease or death confirmation by neutralization

3. Serology – Classical and Newer Techniques

a. Radioimmunoassay

b. Western blot

c. Complement fixation test

diagnostic process