Obtain aliquots of stock solutions of 20 µM bovine serum albumin (BSA), 300 µM 8-anilino1-napthalenesulfonic acid (ANS), and 50 mM phosphate buffer, pH 7.4. From these stocks make 2 ml samples. (hint – use 1 ml BSA, 1 ml ANS + buffer) how to make [BSA]total (µM) 10 and [ANS]total (µM) 5 ?
Obtain aliquots of stock solutions of 20 µM bovine serum albumin (BSA),
300 µM 8-anilino1-napthalenesulfonic acid (ANS), and 50 mM phosphate buffer, pH 7.4. From these stocks make 2 ml samples. (hint – use 1 ml BSA, 1 ml ANS + buffer) how to make [BSA]total (µM) 10 and [ANS]total (µM) 5 ?
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