receiving the plasmid could already be resistant to the antibiotic used. For example, in this

experiment, it is possible that a small fraction of the E. coli cells were already resistant to ampicillin. If true, the colonies seen on the NA-AMP plates in part A would NOT be actual transformants, but rather – just natural variants of the E. coli cells. Due to this concern, experimental controls are performed: in this case, to check on whether there are any ampicillin-resistant E. coli before adding the pGRN plasmid. As you were performing the above transformation experiments, you have followed the same procedure, except you have replaced the pGRN plasmid with water. Predict what you might expect to see if you plate these reactions on NA and NA- AMP plates.

Plate

Colony count

(0, 50, or 10,000)

Colony Color under UV

NA

NA-AMP

                                i.        Which plate, according to your prediction, will have more colonies, NA or NA-AMP? Why?

                               ii.        Do you expect to see colonies on NA-AMP plates? Why or Why not?

                              iii.        What information can you gather by plating E. coli cells on NA and NA-AMP? 

Looking for a Similar Assignment? Order now and Get 10% Discount! Use Coupon Code "Newclient"