Using the following outline, write the lab report using the attached lab report on “Allelopathy”

I. Introduction (Check the word limit)
A. Write about the problem: Kudzu
1. What is it?
2. Why is it a problem?
B. Write about the solution: Allelopathy
1. What is it?
2. How can it help the problem?
3. What plants do it and how do they do it?
C. Write about your specific plant (that might be allelopathic): Black Walnut
1. What is Black Walnut?
2. Is there anything known about Black Walnut and Allelopathy?
D. Write your hypothesis: From the handout
II. Materials and Methods
A. Paraphrase from the handout.
B. Add your own interpretation of how the experiment would have been conducted
III. Results
A. Add your two graphs to your document.
B. Write about what the graphs tell you (as if you had to describe the graphs to someone who could not see them).
C. What are the means and standard deviations?
D. What are the p – values?
IV. Discussion
A. What does you experiment tell you?
B. Is Black Walnut capable of controlling Kudzu?
C. How do you know whether to accept the hypothesis or reject it? The same questions go for the null hypothesis as well.
V. Works Cited

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Bio 123Lab Report“Allelopathy”The AssignmentThis quarter the topic of your second lab report is a phenomenon found in plants calledallelopathy and its application in the prevention of the spread of invasive plant species.Allelopathy, meaningmutual suffering, is essential the effect (either beneficial orharmful) of one plant upon another.Essentially, you can think of it as biological warfarebetween plant species.Typically seen in invasive plant species, allelopathy involves therelease of chemicals (Allelochemicals) from one plant, which negatively impact thegrowth of surrounding plants.Previous experiments investigating allelopathy havefocused on the role of the allelopathic effects of one plant upon seed germination andseedling growth in other plants.The ExperimentJapanese arrowroot,Pueraria lobata, is an invasive plant species introduced into theUnited States as early as 1876.Commonly referred to as “Kudzu,” this plant has beenquickly dominating landscapes, especially in the Southeast, and has become a significantproblem in the agriculture of our country.Many attempts have been made to control itsspread and very little success has been achieved in the prevention of the spread of Kudzuthrough our region.For this experiment, we investigated the potential of allelopathy tobe used as a natural control mechanism for the control of Kudzu.In the state of Georgia, it is illegal to transplant the Kudzu plant.This creates a situationwhere we were required to use a model system (a stand in) in place of the Kudzu for ourlaboratory experiments.In this case, the model system we employed was Kenilworth ivy(Cymbalaria muralis).Kenilworth ivy has similar properties to Kudzu and makes anexcellent experimental subject.We were also required to choose a potentially allelopathic plant to use against Kudzu (or,in the lab, ivy).For this project we chose to use black walnut (Juglans nigra) as ourallelopathic plant. Black walnut has been observed to have allelopathic effects on manyplants; however, a number of plants are thought to be resistant to the affects of blackwalnut.In this experiment, we chose to test the affects of black walnut on Kenilworthivy in order to predict how Kudzu would respond to allelopathy.AllelopathyPage of16

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The HypothesisBased on previously observed allelopathic effects of black walnut on various plants, it ispredicted that exposure to black walnut during growth will reduce germination rate androot length in the invasive plant, Kenilworth Ivy, as compared to plants exposed to wateralone. Additionally, the null hypothesis for this experiment predicts that there will be nodifference in germination rates or root length in ivy plants exposed to black walnut ascompared to plants exposed to water alone.Experimental Design• Briefly, 12000 Kenilworth ivy seeds were purchased from Outsidepride.com• The seeds were divided into two groups (600 in a test group and 600 in a control group)• Within each group of 600, the seeds were further divided into three subgroups (to betested together)• In the control group, three sets of 200 seeds were placed on paper towels or clothtowels to keep moist and watered with water only• In the test group, three sets of 200 seeds were placed on paper towels or cloth towels tokeep moist and watered with a mix of water and ground up black walnut leaves.• In each group the seeds were watered with 10 ml of water or water plus black walnuteach day for two weeks• The ivy seeds were then monitored for signs of germination• After germination, each seed was monitored after an additional week to determine rootlength after germination.AllelopathyPage of26

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