f the ascites method of obtaining monoclonal antibody must be used, all of the following would likely decrease animal pain/distress EXCEPT: Limiting the amount of pristane used to prime the abdomen. Using endpoint criteria tailored to mice with ascites. Increasing the number of abdominal taps performed to prolong animal use. Daily monitoring the degree of abdominal distension and respiratory distress caused by the ascites.

f the ascites method of obtaining monoclonal antibody must be used, all of the following would likely decrease animal pain/distress EXCEPT:
Limiting the amount of pristane used to prime the abdomen.
Using endpoint criteria tailored to mice with ascites.
Increasing the number of abdominal taps performed to prolong animal use.
Daily monitoring the degree of abdominal distension and respiratory distress caused by the ascites.

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