For the DNA sequence shown (PCR sequence 2), choose the primer set
For the DNA sequence shown (PCR sequence 2), choose the primer set
or sets that could be used to amplify a fragment such that you could determine the number of GGCC repeats that occur within the sequence. Note that some primer sequences are listed in 3′ to 5′ orientation.PCR sequence 2.pdf
A.5′-CTAGTTAAGCCATCGATCA -3′ and 5′-CTCTACTGTTTAACCTAGA-3′
B.5′-CTAGTTAAGCCATCGATCA-3′ and 5′-TCTAGGTTAAACAGTAGAG-3′
C.3′-GATCAATTCGGTAGCTAGT-5′ and 5′-TCTAGGTTAAACAGTAGAG-3′
D.5′-GGCCGGCCGGCCGGCCGGCC-3′ and 5′-GGCCGGCCGGCCGGCCGGCC-3′
E.more than one of the above primer sets will work